Part:BBa_G1002:Design

Forward primer for amplifying BioBrick plasmid backbones by PCR (Suffix-f)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 1
  Illegal PstI site found at 15
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 1
  Illegal PstI site found at 15
  Illegal NotI site found at 8
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal suffix found in sequence at 1
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 1
  Illegal PstI site found at 15
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This primer sequence is 1 nucleotide shorter than BBa_G1000.

Source

The BioBrick suffix.